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Hep G2 Cell Line human

85011430, human liver, Epithelial

Synonyme(s) :

HepG2 Cells

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About This Item

Code UNSPSC :
41106514
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

Hep G2 Cell Line human, hepatocyte carcinoma, 85011430

Source biologique

human liver

Mode de croissance

Adherent

Caryotype

Modal no. 55

Morphologie

Epithelial

Produits

Prothrombin, antithromin III, alpha-foetoprotein complement, C3 and C4 activator, fibrinogen

Récepteurs

Not specified

Technique(s)

cell culture | mammalian: suitable

Maladie(s) pertinente(s)

cancer

Conditions d'expédition

dry ice

Température de stockage

−196°C

Origine de la lignée cellulaire

Human Caucasian hepatocyte carcinoma

Description de la lignée cellulaire

The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g. albumin, α2-macroglobulin, α 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. The cells will respond to stimulation with human growth hormone.

Application

Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric assay.[1][2]
Study of hepatocyte function and specific protein expression

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10
THO1: 9
TPOX: 8,9
vWA: 17

Milieu de culture

EMEM (EBSS)+ 2mM Glutamine + 1% Non Essential Amino Acids NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells might take 6 days to form a confluent monolayer without media change if seeded at 1:4. Until then growth occurs mainly in islands.

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Questions

1–7 of 7 Questions  

  1. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

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  2. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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  3. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

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  4. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

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  5. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  6. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

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  7. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

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