T98G Cell Line human

92090213, human brain, Not specified

• Synonyme(s) : T 98 G Cells, T-98G Cells, T98-G Cells
• Code UNSPSC : 41106514

Propriétés

• Nom du produit: T98G Cell Line human, 92090213
• Source biologique: human brain
• Description: Human Caucasian glioblastoma
• Mode de croissance: Adherent
• Caryotype: Hyperpentaploid, modal no. 128-132
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian glioblastoma

Description de la lignée cellulaire

Derived from glioblastoma multiform tumour from a 61-year-old Caucasian male. Indefinite lifespan and anchorage independent but can enter viable G1 arrested state when deprived of serum.

Application

Studies on the mechanism for cessation of proliferation in quiescent cells and cells synchronised in G1 phase.

T98G has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study the efficiency of empty spiracles homeobox 2 (EMX2) in suppressing glioblastoma.

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 13
D5S818: 10,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 17,20

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells may become anchorage-independent if deprived of serum.

Autres remarques

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