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12106C
Sérum de veau fœtal
USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture
Synonyme(s) :
FBS, FCS, sérum, sérums
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About This Item
Source biologique:
bovine fetus
Origine:
USA origin
Stérilité:
sterile-filtered
Conditions d'expédition:
dry ice
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
bovine fetus
Niveau de qualité
Stérilité
sterile-filtered
Gamme de produits
FBS Premier
Composition
hemoglobin, ≤20 mg/dL
Origine
USA origin
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤10 EU/mL endotoxin
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Application
Fetal Bovine Serum (FBS) provides many non-defined growth promoting and survival enhancing factors to cells in culture. FBS, heat inactivated may be used in place of regular FBS wherein the inactivation of the complement system is beneficial to cell growth or function. Heat-inactivated FBS is used to culture Leishmania sp.[1][2]
Notes préparatoires
Prepared by heating at 56°C for 30 minutes.
Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.
Remarque sur l'analyse
- Taux d′endotoxines et d′hémoglobine testés
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
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Certificats d'analyse (COA)
Lot/Batch Number
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Protocoles
Protocole du test au WST-1 pour la mesure de la viabilité, de la prolifération, de l'activation et de la toxicité cellulaires. Instructions de préparation du réactif WST-1 et exemples d'applications. Foire aux questions et guide de résolution des problèmes liés au test au WST-1.
WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.
WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.
Protocole du test au WST-1 pour la mesure de la viabilité, de la prolifération, de l'activation et de la toxicité cellulaires. Instructions de préparation du réactif WST-1 et exemples d'applications. Foire aux questions et guide de résolution des problèmes liés au test au WST-1.
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
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Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
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