09111802

MS-1 cell line

09111802, human skin, mesothelioma. Loose aggregates with irregular outline, no central necrosis.

• Synonyme(s) : MS1 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: MS-1 cell line,
• Source biologique: human skin
• Niveau de qualité: 100
• Forme: liquid
• Mode de croissance: Suspension
• Caryotype: Not determined
• Morphologie: Loosely packed floating aggregates with irregular outline and no central necrosis
• Produits: Cell line is CM2B4/MCV T, AE1/AE2, CK20, EMA, CAM5.2, chromogranin & NSE. Cell line is negative for for CK7, synaptophysin, LCA TTF-1, vimentin and CD99,
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human neuroendocrine skin carcinoma

Description de la lignée cellulaire

MS-1 cells contain a Merkel cell polyomavirus integrated into the genome. See also MKL-1 (Sigma Catalogue number 09111801).

Application

Pathobiology of neuroendocrine skin carcinomas (NSCs) and function of cutaneous Merkel cells. The role of polyomavirus in the development of Merkel cell carcinomas (submitted for publication)

Profil d'ADN

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11,14
D16S539: 9
D5S818: 11
D7S820: 8,9
THO1: 7,9.3
TPOX: 8
vWA: 17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Procédure de repiquage

Seed at 3 x 10⁵ cells/ml in 5% CO₂; 37°C Saturation density is approximately 10⁶ cells/ml.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr Patrick Moore, University of Pittsburgh Cancer Institute, 5117 Centre Avenue Suite 1.8 Pittsburgh PA 15213

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable