Huh-7D 12 Cell Line human

1042712, human liver, Epithelial

• Synonyme(s) : Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: Huh-7D 12 Cell Line human, 01042712
• Source biologique: human liver
• Mode de croissance: Adherent
• Caryotype: That of original Huh-7
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Température de stockage: −196°C

Description

Description générale

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

Origine de la lignée cellulaire

Human hepatocellular carcinoma

Description de la lignée cellulaire

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Application

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

Milieu de culture

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

Autres remarques

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