AGRLE-RO
Roche
Agarose LE
low electroendosmosis
Synonyme(s) :
agarose
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About This Item
Code UNSPSC :
12352200
Produits recommandés
Source biologique
algae (seaweed)
Forme
powder
Conditionnement
pkg of 100 g (11685660001)
pkg of 500 g (11685678001)
Fabricant/nom de marque
Roche
EEO
0.05 -0.13
Température de transition
gel point 36 °C ±1.5
Résistance du gel
>=2,500 g/cm2
Activité étrangère
Dnase, none detected
Rnase, none detected
Conditions d'expédition
ambient
Température de stockage
20-25°C
Description générale
Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.
Application
Agarose LE has been used for:
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.
- analyzing fragments between 0.2 and 15 kb
- analysis of PCR products
- examination of restriction endonucleases
- digests of plasmid, cosmid, and λ phage DNA
- electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
- in combination with ethidium bromide in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.
Qualité
Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose)
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