806595

APN-Azide

95%

• Synonyme(s) : 3-(4-Azidophenyl)propiolonitrile, APN-N₃
• Formule empirique (notation de Hill): C₉H₄N₄
• Numéro CAS: 1643841-88-6
• Poids moléculaire : 168.15
• Code UNSPSC : 12352125
• ID de substance PubChem : 329769021
• Nomenclature NACRES : NA.22

Propriétés

• Niveau de qualité: 100
• Pureté: 95%
• Forme: powder
• Pertinence de la réaction: reagent type: cross-linking reagent
• Pf: 100 °C
• Groupe fonctionnel: azide
• Température de stockage: 2-8°C
• Chaîne SMILES: N#CC#CC1=CC=C(N=[N+]=[N-])C=C1
• InChI: 1S/C9H4N4/c10-7-1-2-8-3-5-9(6-4-8)12-13-11/h3-6H
• Clé InChI: BDLXITYEYYHQNK-UHFFFAOYSA-N

Description

Application

APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.

Standard protein labeling procedure (cysteine labeling)
1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
3. Incubate at room temperature for 2 hours.
4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.
5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.
*Note: avoid thiol-containing buffers.

Informations sur la sécurité

• Pictogrammes: GHS02,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H228,H315,H319,H335
• Conseils de prudence: P210 - P240 - P241 - P261 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 4.1B - Flammable solid hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable