567517-U

Discovery^® HS F5 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

• Sinónimos: Discovery Pentafluorophenyl PFP HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Propiedades

• product name: Columna para HPLC Discovery^® HS F5, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• agency: suitable for USP L43
• product line: Discovery^®
• feature: endcapped
• manufacturer/tradename: Discovery^®
• packaging: 1 ea of
• extent of labeling: 12% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 4.6 mm
• surface area: 300 m²/g
• surface coverage: 4 μmol/m²
• impurities: <10 ppm metals
• matrix: silica gel, high purity, spherical particle platform; fully porous particle
• matrix active group: PFP (pentafluorophenyl) phase
• particle size: 5 μm
• pore size: 120 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Descripción

General description

Características de la Discovery^® HS F5:
• Selectividad única
• Retención similar a la C18 (algunas veces requiere una fase móvil más fuerte)
• Excelente forma del pico
• Estable, escaso sangrado en separaciones de CL/EM (LC/MS)
• Separaciones modulables de 3 a 10μm

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Application

• Determination of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline, 5-hydroxytryptamine and 5-hydroxyindole acetic acid in the neonatal rat brains using high performance liquid chromatography-electrochemical detection.: This study highlights the use of the Discovery^® HS F5 HPLC Column for the sensitive detection of neuroactive compounds in neonatal rat brain samples, advancing our understanding of neurological development and neurotransmitter dynamics (Mao et al., 2009).


• Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase--application to analysis of triamcinolone acetonide.: This research showcases the superior performance of the Discovery^® HS F5 HPLC Column in analyzing corticosteroid formulations, providing insights that could lead to improved analytical methodologies in pharmaceutical analysis (Havlikova et al., 2008).


• Determination of ephedrine alkaloids in Ephedra natural products using HPLC on a pentafluorophenylpropyl stationary phase.: Utilizes the Discovery^® HS F5 HPLC Column for the precise and efficient separation of ephedrine alkaloids, contributing to safer and more effective use of Ephedra in herbal supplements (Pellati and Benvenuti, 2008).


• Fast high-performance liquid chromatography analysis of phenethylamine alkaloids in Citrus natural products on a pentafluorophenylpropyl stationary phase.: Demonstrates the efficiency of the Discovery^® HS F5 HPLC Column in the rapid analysis of phenethylamine alkaloids, enhancing the quality control of Citrus-based dietary supplements (Pellati and Benvenuti, 2007).


• High-performance liquid chromatographic method with UV photodiode-array, fluorescence and mass spectrometric detection for simultaneous determination of galantamine and its phase I metabolites in biological samples.: This study uses the Discovery^® HS F5 HPLC Column to simultaneously quantify galantamine and its metabolites, aiding in the pharmacokinetic profiling of this Alzheimer′s disease medication (Malakova et al., 2007).

Features and Benefits

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany