The proteomics grade Trypsin kit (T7575-1KT) includes 96 individual 1 µg samples, each provided in a single vial.
T7575
Trypsin Protease
Single-use aliquots ensuring consistent proteolytic activity, suitable for mass spectrometry
Sinónimos:
Proteomics grade, Trypsin, TPCK treated
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Comprobar disponibilidad del carrito | $244.000 |
Acerca de este artículo
UNSPSC Code:
12352204
NACRES:
NA.56
Specific activity:
≥10,000 BAEE units/mg protein
Servicio técnico
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Permítanos ayudarleNombre del producto
Trypsin Singles, Proteomics Grade,
grade
Proteomics Grade
Quality Segment
form
ready-to-use solution
specific activity
≥10,000 BAEE units/mg protein
mol wt
average mol wt 23.29 kDa
optimum pH
~8.0
storage temp.
2-8°C
General description
Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.
Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.
Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.
Application
Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry). It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.
Features and Benefits
- Convenient, single-use 1 μg size
- Eliminates repetitive pipetting
- Reductively methylated to minimize autolytic activity
- TPCK treated to quench chymotryptic activity
- Extensively purified
1 of 1
Este artículo | |||
|---|---|---|---|
| description - | description Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, ready-to-use solution | description Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris | description - |
| specific activity ≥10,000 BAEE units/mg protein | specific activity ≥10,000 units/mg protein (Enzymatic activity) | specific activity ≥10,000 units/mg protein | specific activity ≥10,000 U/mg |
| grade Proteomics Grade | grade Proteomics Grade | grade Proteomics Grade | grade Proteomics Grade |
| form ready-to-use solution | form ready-to-use solution | form lyophilized powder | form lyophilized |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt average mol wt 23.29 kDa | mol wt - | mol wt - | mol wt - |
| optimum pH ~8.0 | optimum pH - | optimum pH - | optimum pH - |
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signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Clase de almacenamiento
3 - Flammable liquids
wgk
WGK 2
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How many 1 µg samples are included in the proteomics grade Trypsin kit (T7575-1KT)?
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