SRE0053

Lisostafina from Staphylococcus staphylolyticus

Suitable for manufacturing of diagnostic kits and reagents., lyophilized powder

• Sinónimos: Endopeptidasa glicil-glicina
• Número de CAS: 9011-93-2
• Comisión internacional de enzimas: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556
• UNSPSC Code: 12352204
• NACRES: NA.32

Propiedades

• biological source: bacterial (Staphylococcus sp.)
• Quality Level: 400
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 26,926 Da
• composition: protein, 50-70% biuret
• packaging: pkg of (package size sold on basis of mg protein)
• concentration: 50—70% protein
• suitability: suitable for microbiological tests
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: −20°C

Descripción

General description

Lysostaphin is a glycylglycine endopeptidase. It is an important antibacterial enzyme.

Biochem/physiol Actions

Lysostaphin has the ability to cleave both viable and heat-killed S. aureus cells. It is one of the major antibacterial agents for the staphylococci. Lysostaphin cleaves the cross-linking pentaglycine bridges in the cell walls of staphylococci. Thus it is considered as an antistaphylococcal therapeutic agent.

La lisostafina es una endopeptidasa de cinc con un peso molecular de aproximadamente 25 kDa. Debido a que la lisostafina rompe los enlaces transversales de la poliglicina de la capa de peptidoglucanos de la pared celular de las especies de Staphylococcus , se ha encontrado útil para la lisis celular y también como potencial tratamiento antimicrobiano.
pH de actividad óptimo: ~7,5

Unit Definition

One unit will reduce the turbidity (A₆₂₀) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH7.5 at 37°C in a 6.0ml reaction mixture.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable