SBR00039

Hygromycin B

Min. 95% (HPLC ELSD) Ready Made Solution, 50 mg/mL in water (from Streptomyces hygroscopicus)

• Sinónimos: Hygromycin B solution from Streptomyces hygroscopicus
• Fórmula empírica (notación de Hill): C₂₀H₃₇N₃O₁₃
• Número de CAS: 31282-04-9
• Peso molecular: 527.52
• Beilstein: 6755837
• Número MDL: MFCD06795479
• Código UNSPSC: 51102829
• NACRES: NA.76

Propiedades

• Nombre del producto: Hygromycin B, Min. 95% (HPLC ELSD) Ready Made Solution, 50 mg/mL in water (from Streptomyces hygroscopicus)
• origen biológico: Streptomyces hygroscopicus
• Nivel de calidad: 200
• Ensayo: ≥95%
• Formulario: liquid
• concentración: 50 mg/mL in water (from Streptomyces hygroscopicus)
• espectro de actividad antibiótica: Gram-negative bacteria; Gram-positive bacteria; fungi; viruses
• Modo de acción: protein synthesis | interferes
• temp. de almacenamiento: 2-8°C
• cadena SMILES: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• Clave InChI: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Descripción

Descripción general

Hygromycin B, also known as Hydrovetine and D-streptamine, is a distinctive aminoglycoside antibiotic recognized for its ability to impede the growth of both prokaryotic microorganisms, such as bacteria, and eukaryotic microorganisms like yeasts and mammalian cells. Its mechanism of action involves reinforcing the interaction of peptidyl-tRNA in the ribosomal acceptor ("A") site, ultimately hindering the translocation process mediated by EF-2 (elongation Factor-2). Derived from Streptomyces hygroscopicus, this aminoglycoside antibiotic serves as a valuable selection agent for cells harboring the hygromycin-resistant gene, known as hph or hyg.

Widely utilized for selection and maintenance purposes, Hygromycin B showcases its inhibitory prowess on protein synthesis, contributing to its role in the selection and upkeep of both prokaryotic and eukaryotic cells featuring the hygromycin resistance gene. This antibiotic demonstrates anthelmintic activity in addition to its impact on translational processes in ribosomes across diverse organisms. Its specificity in inhibiting translocation, as well as its influence on decoding fidelity, makes Hygromycin B a valuable tool for the selection of transfected cells containing the Escherichia coli hygromycin resistance gene (hyg or hph) and for maintaining the Hygromycin phenotype in resistant cells. The monophasic inhibition of polypeptide synthesis suggests a single-site binding of Hygromycin B to the ribosome, adding a layer of specificity to its mode of action, making it indispensable for various facets of scientific research in cell biology and biochemical research.

Aplicación

Hygromycin B. has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreadin. as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/m. specifically at 100 μg/mL for prokaryote. 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes

Acciones bioquímicas o fisiológicas

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Características y beneficios

• High-quality antibiotic suitable for multiple research applications
• Ideal for Cell Biology, Metabolomics, and Biochemical research
• Ready-made solution for ease of use
  

Otras notas

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Información de seguridad

• Pictogramas: GHS06
• Palabra de señalización: Danger
• Frases de peligro: H301,H312,H330
• Consejos de prudencia: P260 - P264 - P270 - P280 - P302 + P352 + P312 - P304 + P340 + P310
• Clasificaciones de peligro: Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal
• Código de clase de almacenamiento: 6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable