H7772

Higromicina B from Streptomyces hygroscopicus

lyophilized powder

• Sinónimos: Hygrovetine
• Fórmula empírica (notación de Hill): C₂₀H₃₇N₃O₁₃
• Número CAS: 31282-04-9
• Peso molecular: 527.52
• NACRES: NA.85
• PubChem Substance ID: 24895807
• UNSPSC Code: 51102829
• EC Number: 250-545-5
• MDL number: MFCD06795479

Propiedades

• biological source: Streptomyces hygroscopicus
• Quality Segment: 200
• form: lyophilized powder
• purified by: ion-exchange chromatography
• color: faintly brown to brown, white to beige
• solubility: H₂O: 50 mg/mL
• antibiotic activity spectrum: viruses
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChI key: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Descripción

General description

Chemical structure: aminoglycoside

Application

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes..

Biochem/physiol Actions

Modo de acción: El producto actúa inhibiendo la síntesis de proteínas al inducir la lectura errónea de la plantilla de ARNm en procariotas, con la capacidad de inhibir la traducción.

Espectro antimicrobiano: La higromicina B actúa contra bacterias, hongos y células eucariotas superiores.

Preparation Note

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

Disclaimer

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Información de seguridad

• Pictogramas: GHS06
• Palabra de advertencia: Danger
• Códigos de peligro: H300 + H310 + H330
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• Indicaciones de precaución: P260 - P262 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P304 + P340 + P310