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R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

Sinónimos:

Transfer RNA, tRNA

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About This Item

Número de CAS:
9014-25-9
Número MDL:
MFCD00132200
Código UNSPSC:
12352106
NACRES:
NA.55

grado

for molecular biology

Nivel de calidad

300

tpo

Type V

Formulario

lyophilized powder

actividad específica

15-19 units/mg solid

condiciones de almacenamiento

desiccated

solubilidad

H2O: ~2 mg/mL, clear to hazy

temp. de almacenamiento

2-8°C

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Descripción general

Wheat germ tRNA contains many different specific tRNA molecules.

Aplicación

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Componentes

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Definición de unidad

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000397938
0000347113
0000397938
0000332134
0000294864

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