P3076

Platelet-Derived Growth Factor-AA human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinónimos: PDGF−AA
• Número MDL: MFCD00081862
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Nombre del producto: Platelet-Derived Growth Factor-AA human, PDGF-AA, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• origen biológico: human
• Nivel de calidad: 100
• recombinante: expressed in E. coli
• Ensayo: ≥98% (SDS-PAGE and HPLC)
• Formulario: lyophilized powder
• potencia: 1.0-3.0 ng/mL ED₅₀
• calidad: endotoxin tested
• mol peso: 28.5 kDa (containing 250 amino acid residues)
• envase: pkg of 10 μg; pkg of 50 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• técnicas: cell culture | mammalian: suitable
• impurezas: <1 EU/μg
• Nº de acceso UniProt: P04085
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... PDGFRA(5154)

Descripción

Descripción general

Platelet-derived growth factor (PDGF) is composed two polypeptide subunits of A and B chain. PDGF is produced by a number of cells including smooth muscle cells, endothelial cells and macrophages. The PDGF gene is highly conserved and is mapped to human chromosome 7p22.3. PDGF is structurally and functionally related to vascular endothelial growth factors.

Acciones bioquímicas o fisiológicas

Cell division and relocalization of pulmonary artery smooth muscle cells and fibroblasts is known to be triggered by platelet-derived growth factor (PDGF). Due to its proliferatory function, it is associated with fibroproliferative disorders such as atherosclerosis, pulmonary arterial hypertension and lung fibrosis. PDGF exists both in homo and heterodimeric forms and stimulates α and β-cell surface receptors. Vascular endothelial growth factor expression is regulated by PDGF-AA autocrine signalling and is known to promote tumor angiogenesis in vivo.

Otras notas

Homodimer of the A chain of human PDGF.

Forma física

Sterile-filtered through a 0.2 μm filter. Lyophilized with 10 mM acetic acid.

Nota de análisis

The biological activity is measured by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable