O2260

Mouse Osteopontin

recombinant, expressed in NSO cells, powder, suitable for cell culture

• Sinónimos: Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• Número MDL: MFCD03100698
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• Nombre del producto: Osteopontin from mouse, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
• origen biológico: mouse
• Nivel de calidad: 100
• recombinante: expressed in NSO cells
• esterilidad: sterile
• Ensayo: >95% (SDS-PAGE)
• Formulario: lyophilized powder
• mol peso: 30 kDa by SDS-PAGE (reducing); 65 kDa by SDS-PAGE (reducing) (doublet)
• envase: pkg of 50 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• Nº de acceso UniProt: P10923
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: mouse ... Spp1(20750)

Descripción

Aplicación

Osteopontin from mouse has been used:

• to study its role in a mouse model of preterm brain injury
• as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques
• to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels

Acciones bioquímicas o fisiológicas

Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Nota de análisis

The biological activity is measured by its ability to mediate 293 cell adhesion.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable