Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

L6533

Sigma-Aldrich

Lectin from Dolichos biflorus (horse gram)

biotin conjugate, buffered aqueous solution

Sinónimos:

Dolichos biflorus agglutinin, DBA

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

biotin conjugate

Quality Level

200

form

buffered aqueous solution

composition

Protein, ≥2.5 mg/mL biuret

extent of labeling

≥3 mol biotin per mol lectin

storage temp.

−20°C

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.

Application

Lectin from Dolichos biflorus (horse gram) has been used:
  • to characterize mouse uterine natural killer (uNK) cells
  • in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
  • as a species-specific gonocyte marker for the determination of donor cell colonization
  • to label undifferentiated spermatogonia/cat testicular tissue

Biochem/physiol Actions

Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.
DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Packaging

Sold on the basis of mg active conjugate.

Physical form

contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

B Anne Croy et al.
Methods in molecular biology (Clifton, N.J.), 612, 465-503 (2009-12-25)
The term uterine natural killer (uNK) cell is applied in mice to an abundant but transient NK cell population that undergoes unique, terminal differentiation within embryo implantation sites during endometrial decidualization and pregnancy. In mice, decidualization is induced by attachment
Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Escada-Rebelo S, et al.
Andrology, 4(6), 1159-1168 (2016)
Rajesh Dash et al.
JACC. Cardiovascular interventions, 11(2), 195-205 (2018-01-20)
The study investigated whether a dose response exists between myocardial salvage and the depth of therapeutic hypothermia. Cardiac protection from mild hypothermia during acute myocardial infarction (AMI) has yielded equivocal clinical trial results. Rapid, deeper hypothermia may improve myocardial salvage.
Analysis of uterine natural killer cells in mice
Croy BA, et al.
Natural Killer Cell Protocols, 465-503 (2010)
Association of Dolichos biflorus lectin binding with full differentiation of bovine trophoblast cells
Nakano H, et al.
Reproduction (Cambridge, England), 124(4), 581-592 (2002)
Ver todos los artículos relacionados

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico