Male donors are not screened to be free of HLA antibodies.
H6914
Suero humano
(from male AB clotted whole blood), USA origin, sterile-filtered
Sinónimos:
fuera del coágulo, otc, suero humano, sueros humanos
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Acerca de este artículo
Biological source:
human
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
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Servicio técnico
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Permítanos ayudarlebiological source
human
Quality Segment
sterility
sterile-filtered
composition
Hemoglobin, ≤25 mg/dL
origin
USA origin
technique(s)
cell culture | mammalian: suitable, cell culture | stem cell: suitable
impurities
≤10 EU/mL Endotoxin
shipped in
dry ice
storage temp.
−20°C
Application
El suero AB humano se utiliza en aplicaciones de ingeniería genética, trasplantes y terapia celular para la expansión de células madre mesenquimatosas (MSC) del tejido adiposo o las células del estroma mesenquimatoso de la médula ósea humana; para la generación y el trasplante de injertos normalizados de células madre del epitelio del limbo esclero corneal; para la expansión ex vivo de células NK a partir de sangre periférica en medio de expansión de células madre hematopoyéticas; y para actualización de la tecnología de cultivo de islote humano pretrasplante.
Packaging
El embalaje puede variar para diferentes lotes debido a la escasez de las botellas utilizadas para embalar este producto. La presencia o falta de envoltura retráctil alrededor de la tapa de la botella no afecta a la integridad/esterilidad del producto.
Analysis Note
Analizada la presencia de endotoxinas
Other Notes
No es inusual que nuestros productos séricos humanos estén turbios. Este producto procede de la combinación de sueros y la turbidez indica la presencia de lípidos en el suero. No se dispone de productos séricos humanos transparentes, carentes de lípidos.
Disclaimer
Se analizó cada donante para la hepatitis B y C y se observó que no eran reactivos , y tampoco lo eran para los anticuerpos del virus de la inmunodeficiencia humana (VIH) mediante ELISA. No obstante, los productos de origen humano deben considerarse potencialmente infecciosos y manejarse en consecuencia.
SÓLO PARA INVESTIGACIÓN. Este producto está regulado en Francia cuando está destinado a ser utilizado con fines científicos, como para actividades de importación y exportación (artículo L 1211-1, apartado 2, del Código de Salud Pública). El comprador (es decir, el usuario final) está obligado a obtener una autorización de importación del Ministerio de Investigación de Francia referida al artículo L1245-5-1 II del Código de Salud Pública. Al pedir este producto, usted confirma que ha obtenido la autorización de importación adecuada.
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Este artículo | |||
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable |
| biological source human | biological source human | biological source human | biological source human male plasma (Type AB) |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| composition Hemoglobin, ≤25 mg/dL | composition Hemoglobin, ≤25 mg/dL | composition - | composition hemoglobin, ≤30 mg/dL |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 1
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Have the male donor(s) been screen to be free of HLA antibody (transfusion or transplant recipients?)
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What is the best way to thaw this product? Once thawed, how long can we use it if kept in at 4 oC?
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The recommended storage temperature is -20°C. The following steps confirm the best way to thaw serum:
1. Remove the serum bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
2. Place each container in a 30 to 37 °C water bath or incubator. Excessive temperatures will degrade heat-labile nutrients. If using a
water bath, prevent the bottle caps from being completely submerged.
3. Gently swirl or shake the bottles every 10 - 15 minutes until the serum is completely thawed.
Please note: Multiple thaw/freeze cycles should be avoided as they will hasten serum nutrient degradation and can induce insoluble precipitate formation.Helpful?
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Is this serum manufactured under cGMP?
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This product is not manufactured under cGMP.
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If the serum is being used to complete media for a cell thaw but then the cells are put through a DNA extraction rather than put into culture - would thawing a bottle of serum, aliquoting, and then re-freezing the serum be okay?
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Thawed serum should be mixed thoroughly before being added to cell culture medium. Repeated freeze-thaw cycles may significantly impact serum quality. It is recommended to aliquot the thawed serum for single use and store aliquots at -20 °C. If serum is stored between 2-8 °C, it should be used within 2-4 weeks.
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DOES THIS PRODUCT CONTAIN OESTRADIOL
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The presence of oestradiol in this product is not determined. To see which compounds are tested for, refer to a sample Certificate of Analysis found at: https://www.sigmaaldrich.com/product/sigma/h6914#product-documentation
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At which biosafety level should Human Serum H6914 be handled?
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Blood products should be treated and handled as potentially infectious. It is recommended to use human origin products from normal (non-diseased) donors at Biosafety Level 2 (BSL-2). Although donors may be tested for adventitious infections, as listed on the Certificate of Analysis for the product, no testing can cover all types of infection. Therefore, these products should be treated as potentially infectious and handled accordingly. Laboratory directors and principal investigators are responsible for performing a risk assessment and determining the biosafety level in accordance with applicable national and local regulations.
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Human Serum (Product No. H6914 & Batch No. SLCF0424)의 사용기한은 CoA상에서 2023년 2월 까지인 것으로 확인됩니다. 혹시 해당 batch의 사용기한이 연장될 수도 있을까요?
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The shelf life is not extended for those product which are assigned a firm expiration date. No further testing is performed on these lots.
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Are all your human donors consented?
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All protocols including donor consent are subject to the strict oversight of an Institutional Review Board (IRB) as governed by US law.
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What is the difference between human serum from AB plasma and human serum from AB clotted whole blood?
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Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present. The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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