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G8673

Sigma-Aldrich

β-Glucanase 2, thermostable

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Sinónimos:

β-Glucanase, thermostable

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About This Item

Número de CAS:
62213-14-3
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

liquid

specific activity

≥1.0 units/mg protein

mol wt

38 kDa

concentration

≥20 mg protein/mL (Bradford)

shipped in

wet ice

storage temp.

2-8°C

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Application

β-Glucanase is used to study cell wall modifications and for carbohydrate hydrolysis. It has been used to supplement barley-based diets for poultry and to study the reopening signal conduits and release of dormancy in the Populus species.

Biochem/physiol Actions

β-Glucanase is involved in cell wall modifications and carbohydrate hydrolysis.

Unit Definition

One unit will produce 1 μmole of reducing sugar (measured as glucose) from Beta-glucan per minute at pH 5.8 at 70 °C.

Physical form

Supplied as a solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Päivi L H Rinne et al.
The Plant cell, 23(1), 130-146 (2011-02-02)
In trees, production of intercellular signals and accessibility of signal conduits jointly govern dormancy cycling at the shoot apex. We identified 10 putative cell wall 1,3-β-glucanase genes (glucan hydrolase family 17 [GH17]) in Populus that could turn over 1,3-β-glucan (callose)
T Ribeiro et al.
Poultry science, 90(6), 1245-1256 (2011-05-21)
To improve the nutritive value of barley-based diet for broilers, 2 experiments using 2 different barley lots were performed to evaluate the capacity of a mesophilic cellulase when fused to a β-glucan specific family 11 carbohydrate-binding module. The data revealed
Encarnación Dueñas-Santero et al.
Eukaryotic cell, 9(11), 1650-1660 (2010-09-21)
In yeast, enzymes with β-glucanase activity are thought to be necessary in morphogenetic events that require controlled hydrolysis of the cell wall. Comparison of the sequence of the Saccharomyces cerevisiae exo-β(1,3)-glucanase Exg1 with the Schizosaccharomyces pombe genome allowed the identification
Philip A Lessard et al.
Heliyon, 7(8), e07696-e07696 (2021-08-18)
Carbohydrases are often incorporated into livestock feed as digestive aids to improve animal performance. AC1 is a thermostable carbohydrase with β-1,4-glucanase, endo-cellulase, and cellobiohydrolase activity. AC1 has been expressed in corn, where it accumulates in the grain for easy inclusion

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