TT

92050721, human thyroid, Not specified

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: TT, 92050721
• origen biológico: human thyroid
• modo de crecimiento: Adherent
• cariotipo: Aneuploid karyotype with marker chromosomes
• morfología: Not specified
• productos: Calcitonin, Carcinoembryonic antigen (CEA)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian thyroid medullary carcinoma

Descripción línea celular

Established from a thyroid medullary carcinoma of a 77 year old Caucasian female. Tumourigenic in nude mice.

Aplicación

Tumourigenicity studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 16,18

Medio de cultivo

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Population doubling is very slow, cells can take up to 5 days to reach 50% confluency; media change 2-3 times a week is recommen

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