TM3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse testis
• envase: tube of 5 μg 91060526-DNA-5UG; pkg of vial of cells 91060526-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Not specified
• productos: Prostaglandin F2a
• receptores: Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse BALB/c testis, Leydig cell

Descripción línea celular

Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.

Aplicación

Hormone studies

Medio de cultivo

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37²C.

Otras notas

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