B3528
BSK-H Medium
With sodium bicarbonate and HEPES, liquid, sterile-filtered, suitable for cell culture
Sinónimos:
BSK-H Medium Solution, Barbour-Stoenner-Kelly medium, Cell Culture Medium, Medium for Cell Culture
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
Productos recomendados
Nombre del producto
BSK-H Medium, With sodium bicarbonate, suitable for Borrelia burgdorferi (Qualified)
Nivel de calidad
esterilidad
sterile; sterile-filtered
Formulario
liquid
idoneidad
suitable for Borrelia burgdorferi (Qualified)
aplicaciones
food and beverages
componentes
NaHCO3: 2.2 g/L
glucose: 6.0 g/L (Dextro)
phenol red: 0.02124 g/L
HEPES: 6.0 g/L
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Aplicación
BSK-H medium is a standardized complex medium designed to support the growth of the Lyme disease spirochete, Borrelia burgdorferi. To standardize the procedure for isolating and culturing Lyme disease spirochetes, Pollack, RJ et. al. modified the composition of the medium generally used for this purpose (BSK-II) and developed a system for its distribution. This medium contains no gelatin or agarose, and various components are used in proportions that differ from those in BSK-II. Each of the major proteinacious components was screened by substitution in samples of the complete product. The final medium was evaluated for the capacity to grow related spirochetes including Borrelia burgdorferi N40, Guilford, and JD-1 as well as strains of Borrelia hermsii (HS-1) and of Borrelia coriaceae (CO53). Standardized medium, supplemented with prescreened rabbit serum, facilitates comparison of research results between laboratories and may eventually permit definitive clinical diagnosis of Lyme disease based on demonstration of the pathogen. The standardized medium is designated BSK-H.
Formulation for the growth and expansion of the Lyme desease spirochete, Borrelia burgdorferi
Nota de preparación
1. BSK-H Medium is supplied as a sterile-filtered liquid. After thawing, mix well by inverting the bottle prior to use. If the thawed medium will not be used within a few days it is recommended that the medium be refrozen in working aliquots to avoid repeated free-thaw cycles.
2. Prior to use, we recommend that the medium should be supplemented aseptically with prescreened, sterile-filtered serum to a final concentration of 6%. (See note below)
3. Other supplements may be added aseptically as desired. The nature of the supplement may affect storage conditions and shelf-life of the medium.
NOTE: For best results, serum used to supplement the medium should be prescreened for its ability to support the growth of Borrelia burgdorferi. Prescreened, sterilefiltered Rabbit Serum [Product No. R7136] is available from Sigma.
2. Prior to use, we recommend that the medium should be supplemented aseptically with prescreened, sterile-filtered serum to a final concentration of 6%. (See note below)
3. Other supplements may be added aseptically as desired. The nature of the supplement may affect storage conditions and shelf-life of the medium.
NOTE: For best results, serum used to supplement the medium should be prescreened for its ability to support the growth of Borrelia burgdorferi. Prescreened, sterilefiltered Rabbit Serum [Product No. R7136] is available from Sigma.
Productos recomendados
For best results, serum used to supplement the medium should be prescreened for its ability to support the growth of Borrelia burgdorferi. Prescreened, sterile filtered Rabbit Serum [Product No. R7136] is available from Sigma.
suplemento
Referencia del producto
Descripción
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también adquirido normalmente con este producto
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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R J Pollack et al.
Journal of clinical microbiology, 31(5), 1251-1255 (1993-05-01)
To standardize the procedure for isolating and culturing Lyme disease spirochetes, we modified the composition of the medium generally used for this purpose (BSK-II) and developed a system for its distribution. This medium contains no gelatin or agarose, and various
A G Barbour
The Yale journal of biology and medicine, 57(4), 521-525 (1984-07-01)
The successful isolation and cultivation of Lyme disease spirochetes traces its lineage to early attempts at cultivating relapsing fever borreliae. Observations on the growth of Lyme disease spirochetes under different in vitro conditions may yield important clues to both the
O Brorson et al.
Infection, 25(4), 240-246 (1997-07-01)
The purpose of this study was to evaluate the behaviour of Borrelia burgdorferi under controlled conditions. The occurrence of cystic forms of Borrelia burgdorferi in vitro was noted, and these cysts were able to be transformed to normal, mobile spirochetes.
S Y Sung et al.
Infection and immunity, 68(3), 1319-1327 (2000-02-26)
The ospE gene family of the Lyme disease spirochetes encodes a polymorphic group of immunogenic lipoproteins. The ospE genes are one of several gene families that are flanked by a highly conserved upstream sequence called the upstream homology box, or
Gary P Wormser et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 47(7), 910-914 (2008-08-30)
A potential concern with any serologic test to detect antibodies to Borrelia burgdorferi is whether the epitopes incorporated in the test provide sufficient cross-reactivity to detect infection with all of the pathogenic strains of the species. This is a particular
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