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A2356

Phosphatase, Alkaline from bovine intestinal mucosa

≥5,500 DEA units/mg protein

Phosphatase, Alkaline from bovine intestinal mucosa
1 of 1 reviewers received a sample product or took part in a promotion

Sinónimos:

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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A ustedes/SKUDisponibilidadPrecio
10000 units
Comprobar disponibilidad del carrito
$186.000
50000 units
Comprobar disponibilidad del carrito
$746.000

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Número CAS:
9001-78-9
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.54
Número CE:
3.1.3.1 (BRENDA, IUBMB)
MDL number:
MFCD00131849
Specific activity:
≥5,500 DEA units/mg protein
Concentration:
≥10 mg/mL

$186.000

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form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

Quality Segment

200

specific activity

≥5,500 DEA units/mg protein

mol wt

dimer ~160 kDa

concentration

≥10 mg/mL

storage temp.

2-8°C

General description

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical form

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6

Analysis Note

Package sizes are based on DEA units

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

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Phosphatase, Alkaline from bovine intestinal mucosa ≥5,500 DEA units/mg protein

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Phosphatase, Alkaline from bovine intestinal mucosa BioUltra, ≥5,700 DEA units/mg protein

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Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, ≥4,000 DEA units/mg protein

Sigma-Aldrich

P7923

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from calf intestinal mucosa suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Sigma-Aldrich

79390

Phosphatase, Alkaline from calf intestinal mucosa

specific activity

≥5,500 DEA units/mg protein

specific activity

≥5,700 DEA units/mg protein

specific activity

≥4,000 DEA units/mg protein

specific activity

~2500 U/mg protein (~10 mg/ml)

form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

buffered aqueous glycerol solution

form

solution (clear, colorless)

concentration

≥10 mg/mL

concentration

≥10 mg/mL

concentration

≥10.0 mg/mL

concentration

-

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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