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Merck

A0162

Sigma-Aldrich

Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Goat Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−AP

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:3,000 using IgG, IgA, IgM
western blot: 1:50 dilution

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells.

Application

Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect antibodies from hybridomas by indirect ELISA. The antibody was incubated in microtiter plates for 1 hour at 37°C and detected using p-nitrophenyl phosphate substrate (Sigma). Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect the reactivity of fungal cytoplasmic protein with proteins from the serum of mice infected with yeast-form Y cells by western blot analysis. The antibody was used at a 1:50 dilution.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Infection and immunity, 70(10), 5462-5470 (2002-09-14)
Mice immunized with heat-inactivated, whole yeast-form cells (Y cells) of Candida albicans developed intense, specific humoral and cell-mediated immune responses. However, they were modestly protected against a lethal challenge by the fungus, and their sera did not confer passive protection
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