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122-20

Rat Collagen Type I

from rat tail, liquid, 0.05 mg/mL, suitable for cell culture

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A ustedes/SKUDisponibilidadPrecio
20 mL
Comprobar disponibilidad del carrito
$31.700

Acerca de este artículo

UNSPSC Code:
12352202
NACRES:
NA.75
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
50 μg/mL

$31.700


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Nombre del producto

Rat Tail Collagen Coating Solution (20 ML), Type I, 50 μg/mL, solution, BioReagent, suitable for cell culture

Quality Segment

sterility

sterile; sterile-filtered

product line

BioReagent

form

solution

shelf life

6 mo.

packaging

pkg of 20 mL

concentration

50 μg/mL

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

rat ... Col1a1(29393)

General description

Collagen type I is a fibrillar collagen that consists of two α1 chains and one α2 chain twisted together to form a triple helix structure. It is the predominant protein in vertebrates and plays a significant role in the extracellular matrices present in various tissues such as bones, tendons, skin, ligaments, cornea, and other types of connective tissue between cells. Rat Tail Collagen Coating Solution (20 ML) enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. It is commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.
This collagen solution is prepared in 0.02 M Acetic Acid at 50 ug/ml and sterile filtered.

Application

Rat Tail Collagen Coating Solution (20 ML) is suitable for use in cell culture to study the growth, differentiation, and migration of cells. The rat-tail collagen I coating solution has been used to coat 96-well plates, providing a suitable surface for the adhesion and growth of primary keratinocytes in cell culture experiments.[1]

Preparation Note

To prepare the tissue culture ware, apply Rat Tail Collagen Solution at a culture surface ratio of 5 ug/cm2. Here are some examples:
  • For a T-25 flask, use 125 ug (2.5 ml) of the solution.
  • For each well of a 24-well plate, use 10.5 ug (210 ul) of the solution.
After applying the solution, incubate the coated tissue culture ware at 37oC for 2 hours. Carefully remove the Collagen Coating Solution by aspiration. Rinse the coated surface twice with PBS. The coated tissue culture ware can be used immediately or air-dried and stored at 4oC for up to one week.

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Este artículo
125-50C7661C3867
technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile; sterile-filtered

sterility

-

sterility

-

sterility

sterile-filtered

concentration

50 μg/mL

concentration

-

concentration

-

concentration

>0.5 mg/mL Biuret

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

form

solution

form

liquid

form

powder

form

liquid

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice


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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. Need to dilute with acetic acid or we can use directly

    1 answer
    1. 122-20 is provided ready to use at a concentration of 50ug/mL. No additional dilution is recommended unless required by your application.

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