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Merck

SE100

Sigma-Aldrich

Silver Enhancer Kit

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

form

liquid

Quality Level

usage

sufficient for ≥100 slides staining and fixing

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Application

Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.

Features and Benefits

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Packaging

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.

Principle

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Solo componentes del kit

Referencia del producto
Descripción

  • Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • S5020Silver enhancer solution A 100 mLSDS

  • S5145Silver enhancer solution B 100 mLSDS

pictograms

Health hazardEnvironment

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable


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Tumor hypoxia is a well-recognized driver of resistance to traditional cancer therapies such as chemotherapy and radiation therapy. We describe development of a new nanoconstruct composed of gold nanorods (GNRs) conjugated to carbonic anhydrase IX (CAIX) antibody that specifically binds

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