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A0576

Agarosa

Low EEO

Sinónimos:

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE

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A ustedes/SKUDisponibilidadPrecio
25 g
Comprobar disponibilidad del carrito
$152.000
100 g
Comprobar disponibilidad del carrito
$443.000

Acerca de este artículo

Número CAS:
UNSPSC Code:
41105317
PubChem Substance ID:
EC Number:
232-731-8
NACRES:
NA.21
MDL number:

$152.000


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Quality Segment

type

Type I-B

form

powder

technique(s)

electrophoresis: suitable

impurities

≤7% water

ash

≤0.25%

turbidity

≤4 NTU (1.5% gel)

EEO

≤0.12

mp

86.5-89.5  °C

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

gel strength

≥1800 g/cm2 (1% gel), ≥3200 g/cm2 (1.5% gel)

anion traces

sulfate (SO42-): ≤0.12%

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

General description

Agarose, a natural polymer is obtained from sea algae. This neutral linear polysaccharide is made up of d-galactose and 3,6-anhydro-l-galactose. These are connected alternatively with the help of glycosidic bonds. Agarose has high significance in gel electrophoresis and gel chromatography.

Application

Agarose has been used:
  • to form the photosynthetic slab
  • in comparative study, to compare it with native agar, and agarose of Gracilaria dura
  • to estimate the amino acid concentrations in agarose and agar samples
  • as immobilisation substrates for enzyme immobilisation
  • for separating high molecular weight nucleic acids at low gel concentrations

Biochem/physiol Actions

Agarose is used as bioink due because of its characteristic properties such as, biocompatibility, mechanical strength and gelling ability at low temperature.

Analysis Note

A continuación se presenta una lista de las propiedades asociadas a nuestras agarosas:
Contenido de sulfatos - utilizados como un indicador de pureza, ya que los sulfatos constituyen el principal grupo iónico presente.
Fuerza del gel - la fuerza que debe aplicarse a un gel para provocar su fractura.
Punto de gelificación - la temperatura a la cual una disolución acuosa de agarosa forma un gel cuando se enfría. Las disoluciones de agarosa exhiben histéresis en la transición de líquido a gel - es decir, su punto de gelificación no es el mismo que su temperatura de fusión.
Electroendosmosis (EEO) - movimiento de líquido a través del gel. En un gel de agarosa, los grupos aniónicos están pegados a la matriz y no pueden moverse, pero los contra-cationes disociables pueden migrar en la matriz hacia el cátodo, dando lugar a EEO. Dado que el movimiento electroforético de los biopolímeros suele ser en dirección al ánodo, la EEO puede alterar las separaciones debido a la convección interna.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

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Este artículo
A0169A6013A9539
EEO

≤0.12

EEO

0.09-0.13

EEO

0.09-0.13

EEO

0.09-0.13

form

powder

form

powder

form

powder

form

powder

gel strength

≥1800 g/cm2 (1% gel), ≥3200 g/cm2 (1.5% gel)

gel strength

≥1200 g/cm2 (1% gel)

gel strength

≥1200 g/cm2 (1% gel)

gel strength

≥1200 g/cm2 (1% gel)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

impurities

≤7% water

impurities

≤7% water

impurities

≤10% water

impurities

≤10% moisture content


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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