71340-M

pCDFDuet-1 DNA - Novagen

• Sinónimos: Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA
• Código UNSPSC: 41106602
• NACRES: NA.51

Propiedades

• Formulario: liquid
• Nivel de calidad: 100
• fabricante / nombre comercial: Novagen^®
• condiciones de almacenamiento: OK to freeze
• Promotor: Promoter name: T7
• Selección: spectinomycin
• temp. de almacenamiento: −70°C

Descripción

Descripción general

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.

Aplicación

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.[1][2][3]

Advertencia

Toxicity: Standard Handling (A)

Información legal

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable