34115-M

N-Ethylmaleimide

Sulfhydryl alkylating reagent that inhibits H⁺-ATPase and suppresses the short circuit current (IC₅₀ = 22 µM) in pancreatic duct cells.

• Sinónimos: N-Ethylmaleimide, NEM
• Fórmula empírica (notación de Hill): C₆H₇NO₂
• Número de CAS: 128-53-0
• Peso molecular: 125.13
• Número MDL: MFCD00005509
• Código UNSPSC: 12352111

Propiedades

• descripción: Merck USA index - 14, 3822
• Nivel de calidad: 100
• Ensayo: ≥98% (HPLC)
• Formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• color: white
• solubilidad: ethanol: 5 mg/mL
• temp. de almacenamiento: 10-30°C
• cadena SMILES: N1(C(=O)C=CC1=O)CC
• InChI: 1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3
• Clave InChI: HDFGOPSGAURCEO-UHFFFAOYSA-N

Descripción

Descripción general

Sulfhydryl alkylating reagent that inhibits H⁺-ATPase and suppresses the short circuit current (IC₅₀ = 22 µM) in pancreatic duct cells. Inactivates NADP-dependent isocitrate dehydrogenase. Also a potent inhibitor of both Mg²⁺ and Ca²⁺/Mg²⁺-stimulated DNA fragmentation in rat liver nuclei. Stimulates arachidonic acid release through activation of PLA₂ in endothelial cells.

Sulfhydryl alkylating reagent. Has been used for blocking sulfhydryl containing reagents which interfere with glucose oxidase assays. Inactivates NADP-dependent isocitrate dehydrogenase. Endonuclease inhibitor. Also a potent inhibitor of both Mg²⁺ and Ca²⁺/Mg²⁺-stimulated DNA fragmentation in rat liver nuclei.

Acciones bioquímicas o fisiológicas

Primary Target
H+-ATPase

Target IC₅₀: 22 µM in suppressing short circuit current

Advertencia

Toxicity: Corrosive (L)

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Otras notas

Huang, Y.C., et al. 1995. Arch. Biochem. Biophys. 316, 485.
Neve, E.P.A., et al. 1995. Biochem. Pharmacol. 49, 57.
Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.
Banger, K.K., et al. 1993. Biochem. J. 290, 199.
Mattingly, J.R., Jr., et al. 1993. J. Biol. Chem. 268, 3925.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS06,GHS05
• Palabra de señalización: Danger
• Frases de peligro: H300,H311,H314,H317
• Consejos de prudencia: P260 - P270 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
• Código de clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): 163.2 °F - closed cup
• Punto de inflamabilidad (°C): 72.9 °C - closed cup