208748

Calpain-1 Substrate, Fluorogenic

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

• Sinónimos: Calpain-1 Substrate, Fluorogenic, μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
• Fórmula empírica (notación de Hill): C₇₉H₉₅N₁₃O₁₉S₂
• Peso molecular: 1594.81
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥95% (HPLC)
• Formulario: lyophilized solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; desiccated (hygroscopic); protect from light
• color: red
• solubilidad: DMSO: 1 mg/mL
• fluorescencia: λ_ex ~490 nm; λ_em ~518 nm
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence.

Acciones bioquímicas o fisiológicas

Cell permeable: no

Primary Target
A sensitive and specific substrate for calpain-1

Product does not compete with ATP.

Reversible: no

Envase

Packaged under inert gas

Advertencia

Toxicity: Standard Handling (A)

Secuencia

H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH

Forma física

Supplied as a trifluoroacetate salt.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Otras notas

Mittoo, S., et al. 2003. Anal. Biochem.319, 234.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable