14-452-M

PDK1 Protein, active, 10 µg

Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.26

Propiedades

• origen biológico: human
• Nivel de calidad: 100
• recombinante: expressed in baculovirus infected Sf21 cells
• Línea del producto: Upstate^®
• actividad específica: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• mol peso: M_w 59 kDa
• purificado por: (Ni²⁺/NTA-agarose)
• reactividad de especies: human
• fabricante / nombre comercial: Upstate^®
• técnicas: activity assay: suitable (kinase)
• nº de acceso: (FUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. ); NM_002613.3
• Nº de acceso NCBI: NM_002613.3; NM_031268.4
• Nº de acceso UniProt: O15530
• Información sobre el gen: human ... PDPK1(5170)

Descripción

Descripción general

N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end.

Acciones bioquímicas o fisiológicas

Protein Target: PDK1

Target Sub-Family: AGC

Envase

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-452M when ordering the 250 µg size.

Calidad

Routinely evaluated by phosphorylation of PDKtide

Descripción de destino

PDK1

Forma física

50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol

Ni²⁺/NTA-agarose

Almacenamiento y estabilidad

6 months at -70°C

Otras notas

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317
• Consejos de prudencia: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Clasificaciones de peligro: Skin Sens. 1
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°C): Not applicable