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Key Documents

04-811

Sigma-Aldrich

Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal

culture supernatant, Upstate®

Sinónimos:

NOS III, endothelial nitric oxidase synthase, endothelial nitric oxide synthase, nitric oxide synthase 3 (endothelial cell)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

monoclonal

species reactivity

bovine

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pThr495)

Gene Information

mouse ... Nos3(287024)

Specificity

Predicted cross-reactivity with human, rabbit, mouse and rat
Recognizes phosphorylated eNOS/NOS III.

Immunogen

Peptide containing the sequence KK[pT]FK in which pT corresponds to phosphothreonine at residue 495 of human eNOS/NOS III

Application

This Anti-phospho-eNOS/NOS III (Thr495) Antibody is validated for use in WB for the detection of phospho-eNOS/NOS III (Thr495).
Western Blot Analysis:
A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.

Quality

routinely evaluated by immunoblot on fetal bovine heart endothelial cell lysate

Target description

135 kDa

Linkage

Replaces: 05-811

Physical form

Cultured supernatant in 0.05% sodium azide

Analysis Note

Control
Fetal bovine heart endothelial cell lysate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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The Journal of pharmacology and experimental therapeutics, 351(3), 500-509 (2014-09-23)
At the cardiovascular level, nitric oxide (NO) controls smooth muscle functions, maintains vascular integrity, and exerts an antihypertensive effect. Metal-nonoates are a recently discovered class of NO donors, with NO release modulated through the complexation of the N-aminoethylpiperazine N-diazeniumdiolate ligand

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