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RAB0675

Human Hemoglobin ELISA Kit

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1 kit
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CLP 561,000

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

CLP 561,000


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species reactivity

human

technique(s)

ELISA: suitable

assay range

inter-assay cv: <10%
intra-assay cv: <12%

shipped in

wet ice

storage temp.

−20°C

General description

The antibody pair provided in this kit recognizes Human Hemoglobin. Hemoglobin (Hb) is an iron-containing metalloprotein, which is highly expressed in red blood cells. In addition, it is also expressed in nonerythroid cells such as epithelial cells of different origin. Hb is a tetramer composed of two α-globin chains and two β-globin chains. α-globin and β-globin chains is encoded by a gene mapped to human chromosome 16 and 11, respectively.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Hemoglobin (Hb) serves as the primary means of oxygen transport in vertebrates. Hb exhibits antioxidant and antimicrobial activity. Alterations in blood hemoglobin levels occur in many diseases such as anemia and polycythemia. In addition, abnormalities in Hb concentration might be implicated in pathologies associated with various respiratory diseases, such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive lung disease (COPD).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB1038RAB0503RAB0188
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

assay range

inter-assay cv: <10%
intra-assay cv: <12%

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 24 pg/ml

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 3.28-800 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.5 pg/mL
standard curve range: 0.55-400 pg/mL

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Certificates of Analysis (COA)

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Questions

  1. Could you please confirm if the RAB0675 kit is capable of detecting total hemoglobin? Additionally, I would like to know the types of capture and detection antibodies used in this kit.

    1 answer
    1. The capture and detection antibodies are monoclonal Mouse IgG. The hemoglobin antibody was raised in mouse using human hemoglobin as the immunogen. The kit will only be able to detect hemoglobin in serum/plasma, so it cannot be used to detect total hemoglobin in whole blood.

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