MSP01
Membrane Scaffold Protein 1D1
recombinant, expressed in E. coli, buffered aqueous solution
Synonym(s):
Membrane Scaffold Protein 1D1, MSP1D1, MSP1T2
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 mg | Check Cart for Availability | CLP 424,000 |
About This Item
NACRES:
NA.26
UNSPSC Code:
12352200
Form:
buffered aqueous solution
Assay:
≥90% (SDS-GE)
Recombinant:
expressed in E. coli
Mol wt:
24,661.6 Da
recombinant
expressed in E. coli
Quality Level
assay
≥90% (SDS-GE)
form
buffered aqueous solution
mol wt
24,661.6 Da
solubility
water: soluble
shipped in
dry ice
storage temp.
−20°C
General description
Research area: Cell signaling. Nanodiscs are non-covalent structures that contain a phospholipidbilayer and a membrane scaffold protein (MSP), a genetically engineeredprotein, that mimics the function of Apolipoprotein A-1 (ApoA-1). The firstMSP, MSP1, was engineered with its sequence based on the sequence of A-1, butwithout the globular N-terminal domain of native A-1. The Membrane ScaffoldProtein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in theHelix 1 portion (referred to as “H0.5” in the accompanying figure) of theoriginal MSP1 sequence.It consists of an N-terminal histidine-tagged protein with a tobaccoetch virus (TEV) protease cleavage site that is present between the proteinsequence and histidine-tag.Nano-disctechnology is an approach that allows aqueous soluble membrane proteins in anative-like bilayer environment that enables the membrane proteins to remainactive and stable.
Application
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
Nanodisc soluble lipid bilayer systems have proven to be a widely applicable means for rendering membrane proteins soluble in aqueous solutions in a native-like bilayer environment where they remain monodisperse and active. The critical component of nanodiscs is the encircling amphipathic helical protein belt (membrane scaffold protein).
The nanodisc system has been employed to incorporate a wide variety of proteins including GPCRs, P450s, bacteriorhodopsin, coagulation factors, cholera toxin, TAR receptor and aromatase.
For an extensive list of citations and protocols visit the Sligar Lab Website at; sligarlab.life.uiuc.edu/nanodisc.html
Biochem/physiol Actions
Generates Nanodiscs ~9.7 nm in diameter
MSP1D1 can self-assembleinto discoidal nanoparticles known as nano-discs. These nano-discs may be used inthe field of medicine, biotechnology, and membrane protein studies.[1]
Legal Information
Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
- 7,691,414 Membrane scaffold proteins
- 7,662,410 Membrane scaffold proteins and embedded membrane proteins
- 7,622,437 Tissue factor compositions and methods
- 7,592,008 Membrane scaffold proteins
- 7,575,763 Membrane scaffold proteins and tethered membrane proteins
- 7,083,958 Membrane scaffold proteins
- 7,048,949 Membrane scaffold proteins
Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
- 7,691,414 Membrane scaffold proteins
- 7,662,410 Membrane scaffold proteins and embedded membrane proteins
- 7,622,437 Tissue factor compositions and methods
- 7,592,008 Membrane scaffold proteins
- 7,575,763 Membrane scaffold proteins and tethered membrane proteins
- 7,083,958 Membrane scaffold proteins
- 7,048,949 Membrane scaffold proteins
1 of 1
This Item | |||
|---|---|---|---|
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| assay ≥90% (SDS-GE) | assay ≥90% (SDS-GE) | assay ≥90% (SDS-GE) | assay - |
| mol wt 24,661.6 Da | mol wt - | mol wt 32,599.6 Da | mol wt Mw 32599.98 by amino acid sequence |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder |
| shipped in dry ice | shipped in ambient | shipped in ambient | shipped in - |
| solubility water: soluble | solubility - | solubility water: soluble | solubility - |
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.
Related Content
Instructions
Christopher I Cazzonelli et al.
eLife, 9 (2020-02-01)
Carotenoids are a core plastid component and yet their regulatory function during plastid biogenesis remains enigmatic. A unique carotenoid biosynthesis mutant, carotenoid chloroplast regulation 2 (ccr2), that has no prolamellar body (PLB) and normal PROTOCHLOROPHYLLIDE OXIDOREDUCTASE (POR) levels, was used
Ramona Faas et al.
Biotechnology reports (Amsterdam, Netherlands), 17, 45-48 (2018-01-31)
Membrane scaffold proteins (MSPs) are synthetic derivatives of apolipoprotein A-I, a major protein component of human high-density lipoprotein complexes. The most common among these is the variant MSP1D1, which has been in the focus of research on membrane mimetics in
Benjamin J Cole et al.
PLoS biology, 15(9), e2002860-e2002860 (2017-09-25)
Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MSP01-5MG | 04061831159622 |