This product is an ammonium sulfate suspension of Myokinase. This information may be determined by values reported in the Certificate of Analysis. A survey of recent lots indicates the an average volume of 160 uL for the 1KU package size, 400 uL for the 2.5KU package size, 800 uL for the 5KU package size, and 1.6 mL for the 10KU package size. The enzyme activity may range from 1500 - 3000 units per milligram protein and the protein concentration may range from 1 - 10 mg/mL. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/m3003#product-documentation
M3003
Myokinase from rabbit muscle
ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)
Synonym(s):
ATP: AMP phosphotransferase, Adenylate Kinase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1000 units | Check Cart for Availability | CLP 64,900 | |
| 2500 units | Check Cart for Availability | CLP 113,000 | |
| 5000 units | Check Cart for Availability | CLP 189,000 | |
| 10000 units | Check Cart for Availability | CLP 328,000 |
About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-743-3
NACRES:
NA.54
MDL number:
Specific activity:
1,500-3,000 units/mg protein (biuret)
Biological source:
rabbit muscle
biological source
rabbit muscle
Quality Level
form
ammonium sulfate suspension
specific activity
1,500-3,000 units/mg protein (biuret)
mol wt
21 kDa
foreign activity
3-phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase and pyruvate kinase ≤0.01%
storage temp.
2-8°C
General description
Research area: Cell Signaling
Myokinase, also called Adenylate kinase or ADK, is a phosphotransferase enzyme. It belongs to the family of nucleoside monophosphate kinases. Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator.
Myokinase, also called Adenylate kinase or ADK, is a phosphotransferase enzyme. It belongs to the family of nucleoside monophosphate kinases. Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator.
Application
Myokinase has been used:
- in a study to assess its reaction with the mercurials p-hydroxymercuribenzoate and p-mercuribenzenesulfonate [1]
- in a study to investigate the ligand-binding properties of its peptide fragments [2]
- as one of the components in the ATP generation solution for the quantification of 3’3’-c-di-AMP using an AMP-Luciferase assay [3]
Biochem/physiol Actions
Myokinase or adenylate kinases, play a crucial role in regulating energy metabolism within cells and balancing adenine nucleotide ratios in different cellular compartments.
Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol.
Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP
Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol.
Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP
Rabbit muscle myokinase can be inactivated by treatment with dithiobis-(2-nitrobenzoic) acid which alters methionine residues critical to myokinase activity. [4]
Physical form
Suspension in 3.2 M (NH4)2SO4 solution containing 0.001 M EDTA, pH 6.0
Other Notes
One unit will convert 2.0 μmoles of ADP to ATP+AMP per min at pH 7.6 at 37°C.
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This Item | |||
|---|---|---|---|
| specific activity 1,500-3,000 units/mg protein (biuret) | specific activity 350-600 units/mg protein | specific activity 350-600 units/mg protein | specific activity ≥4,000 units/mg protein |
| biological source rabbit muscle | biological source rabbit muscle | biological source rabbit muscle | biological source rabbit muscle |
| form ammonium sulfate suspension | form ammonium sulfate suspension | form lyophilized powder | form ammonium sulfate suspension |
| mol wt 21 kDa | mol wt 237 kDa | mol wt 237 kDa | mol wt calculated mol wt 53.2 kDa |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| foreign activity 3-phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase and pyruvate kinase ≤0.01% | foreign activity lactic dehydrogenase, creatine phosphokinase, and myokinase ≤0.01%, phosphoglucomutase ≤0.05% | foreign activity lactic dehydrogenase, creatine phosphokinase, phosphoglucomutase, and myokinase ≤0.01% | foreign activity 3-phosphoglyceric phosphokinase ≤0.01%, glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001% |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Related Content
The sulfhydryl groups of rabbit muscle adenosine triphosphate-adenosine monophosphate phosphotransferase. Activity of enzyme treated with mercurials.
L F Kress et al.
The Journal of biological chemistry, 241(10), 2293-2300 (1966-05-25)
The many isoforms of human adenylate kinases
Panayiotou C, et al.
The International Journal of Biochemistry & Cell Biology, 75-83 (2014)
Secretion from dense granules: luminescence method for adenine nucleotides.
M Fred Heath
Methods in molecular biology (Clifton, N.J.), 272, 89-93 (2004-07-01)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M3003-1KU | 04061832924526 |
| M3003-2.5KU | 04061834046295 |
| M3003-10KU | 04061832924519 |
| M3003-5KU | 04061832924540 |
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