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E0760

Endothelial cell growth supplement from bovine pituitary

Bioreagent, powder, suitable for cell culture

Synonym(s):

ECGS, Endothelial Mitogen

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Pack SizeSKUAvailabilityPrice
15 mg

Available to ship TODAYfromPUDAHUEL

CLP 159,000

About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
Biological source:
bovine pituitary glands
Form:
powder
Impurities:
≤20 EU/vial (LAL test)

CLP 159,000


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Product Name

Endothelial cell growth supplement from bovine pituitary, ECGS, BioReagent, suitable for cell culture

biological source

bovine pituitary glands

Quality Level

product line

BioReagent

form

powder

potency

2-300 μg/mL ED50/EC50

quality

endotoxin tested

packaging

pkg of 15 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤20 EU/vial (LAL test)

color

brown to tan

solubility

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

storage temp.

2-8°C

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:
  • as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
  • as a supplement in medium 199 for endothelial cell culture
  • as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
  • as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

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This Item
E2759P5208E1388
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

bovine pituitary glands

biological source

bovine neuroblastoma

biological source

human

biological source

human

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

lyophilized powder

form

lyophilized powder

impurities

≤20 EU/vial (LAL test)

impurities

≤20 EU/vial (LAL test)

impurities

<1 EU/mg

impurities

≤1.0 EU/μg protein Endotoxin level (LAL test)

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)


pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Repr. 2

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Guihua Wu et al.
Pharmaceutical biology, 59(1), 1388-1401 (2021-10-20)
Genistein (Gen) has shown protective effects against ageing process. To explore the role of Gen on the senescence of H2O2-induced human umbilical vein endothelial cells (HUVECs) and investigate the possible mechanism. HUVECs were treated with different concentrations of H2O2 (50
TGF-beta 1 released from activated platelets can induce TNF-stimulated human brain endothelium apoptosis: a new mechanism for microvascular lesion during cerebral malaria
Wassmer SC, et al.
Journal of Immunology, 176(2), 1180-1184 (2006)
Surface staining and cytotoxic activity of heat-shock protein 60 antibody in stressed aortic endothelial cells.
Xu Q, et al.
Circulation Research, 75(6), 1078-1085 (1994)



Global Trade Item Number

SKUGTIN
E0760-15MG04061835514120

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