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| Size/SKU | Availability | Price |
|---|---|---|
0.1 mg | Estimated to ship onJune 18, 2026fromPUDAHUEL | CLP 589,000 |
About This Item
NACRES:
NA.25
UNSPSC Code:
12352202
CLP 589,000
Estimated to ship onJune 18, 2026Details
Quality Segment
form
liquid
usage
1000 uses (0.5 mg size), 200 uses (0.1 mg size)
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Preparation Note
Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.
Other Notes
Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
| form liquid | form liquid | form liquid | form liquid |
| Quality Level 100 | Quality Level 100, 200 | Quality Level 100, 200 | Quality Level 100, 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| usage 1000 uses (0.5 mg size) | usage 200 uses (0.1 mg size), 40 uses (20 μg size) | usage 100 uses (0.1 mg size), 500 uses (0.5 mg size) | usage 200 uses (0.1 mg size), 40 uses (20 μg size) |
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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