D7876
Diamine Oxidase from porcine kidney
≥0.05 unit/mg solid
Synonym(s):
Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing)
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| Size/SKU | Availability | Price |
|---|---|---|
250 mg | Check Cart for Availability | CLP 73,600 |
1 g | Check Cart for Availability | CLP 236,000 |
5 g | Check Cart for Availability | CLP 713,000 |
10 g | Check Cart for Availability | CLP 1,240,000 |
About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-613-6
NACRES:
NA.54
MDL number:
Specific activity:
≥0.05 unit/mg solid
Biological source:
Porcine kidney
biological source
Porcine kidney
Quality Segment
form
solid
specific activity
≥0.05 unit/mg solid
mol wt
170 kDa
solubility
100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL
foreign activity
monoamine oxidase (benzylamine substrate) ≤1%
storage temp.
−20°C
General description
Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper. The molecular mass of the enzyme is found to be 170 kDa.[1] The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A.[2] Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.
Application
Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase.
Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.
Biochem/physiol Actions
Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism. Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.
Other Notes
One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥0.05 unit/mg solid | specific activity ≥1.5 units/mg solid | specific activity ≥10 units/mg protein (Bradford) | specific activity ≥12 units/mg protein (biuret) |
| biological source Porcine kidney | biological source - | biological source - | biological source Porcine kidney |
| form solid | form powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility 100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL | solubility - | solubility - | solubility - |
| mol wt 170 kDa | mol wt - | mol wt - | mol wt 320 kDa |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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