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CS0170

Thioredoxin Reductase Assay Kit

1 kit sufficient for 100 assays (1 mL)

Synonym(s):

Thioredoxin Reductase Activity Kit

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1 kit
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CLP 797,000

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

CLP 797,000

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Quality Level

200

usage

 kit sufficient for 100 assays (1 mL)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)

General description

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H+ ↔ 2 TNB + NADP+

Application

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Features and Benefits

  • An easy and simple method
  • The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
  • The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
  • The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

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Thioredoxin Reductase Assay Kit 1 kit sufficient for 100 assays (1 mL)

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Gene Information

human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)

Gene Information

human ... HMGCR(3156)

Gene Information

human ... GSR(2936)

Gene Information

human ... CS(1431)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

usage

 kit sufficient for 100 assays (1 mL)

usage

sufficient for 100 assays (200 μL), sufficient for 30 assays (1 mL)

usage

sufficient for 700 assays

usage

 kit sufficient for 100 reactions (using a 1 ml cuvette),  kit sufficient for 480 reactions (using 96 multiwell plates)

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−70°C

storage temp.

2-8°C

storage temp.

−20°C

Kit Components Only

Product No.
Description
  • Assay Buffer 5x for Thioredoxin Reductase 30 mL
  • Thioredoxin Reductase 10 μg
  • Thioredoxin Reductase Inhibitor Solution .05 mL
  • 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
  • NADPH 25 mg
  • Dimethyl Sulfoxide (DMSO) 7.5 mL

signalword

Warning

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F

flash_point_c

87 °C

pictograms

Health hazardExclamation mark
GHS08,GHS07

Hazard Classifications

Eye Irrit. 2 - Repr. 2 - STOT SE 2

target_organs

Eyes,Central nervous system

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000541776
0000541776
0000330984
0000330984
0000274428

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Global Trade Item Number

SKUGTIN
CS0170-1KT04061833553077

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