This product is shipped at ambient temperature. This material is lyophilized from a solution containing sodium phosphate, pH 6.5. It may be reconstituted in approximately 80 ul water per 25 ug vial. Activation is not necessary.
C8696
Cathepsin D from human liver
lyophilized powder, ≥250 units/mg protein (E1%/280)
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 μg | Check Cart for Availability | CLP 300,000 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Specific activity:
≥250 units/mg protein (E1%/280)
form
lyophilized powder
Quality Level
specific activity
≥250 units/mg protein (E1%/280)
mol wt
~45 kDa
color
white
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CTSD(1509)
General description
Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.
Application
Cathepsin D from human liver has been used:
- in β-secretase activity assay
- for enzymatic degradation of amyloid β 1-42[1]
- in microinjection of human foreskin fibroblasts
Biochem/physiol Actions
Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.
Endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts.
Physical form
Powder containing sodium phosphate buffer salt.
Other Notes
Contains α, β and γ isoenzymes as observed by isoelectric-focusing.
One unit will produce an increase in A280 of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information human ... CTSD(1509) | Gene Information human ... CTSD(1509) | Gene Information cow ... CTSD(282883) | Gene Information human ... CTSB(1508) |
| specific activity ≥250 units/mg protein (E1%/280) | specific activity - | specific activity ≥2.0 units/mg protein | specific activity ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.) |
| form lyophilized powder | form lyophilized | form lyophilized powder | form buffered aqueous solution |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| mol wt ~45 kDa | mol wt calculated mol wt 43.6 kDa, observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.) | mol wt ~45 kDa | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Platelet membrane beta-secretase activity in mild cognitive impairment and conversion to dementia: a longitudinal study
McGuinness B, et al.
Journal of Alzheimer'S Disease, 49(4), 1095-1103 (2016)
Aspartic Proteinases Physiology and Pathology (1995)
Jeane do Nascimento Moraes et al.
The journal of venomous animals and toxins including tropical diseases, 28, e20220002-e20220002 (2022-11-22)
Cathepsin D (CatD) is a lysosomal proteolytic enzyme expressed in almost all tissues and organs. This protease is a multifunctional enzyme responsible for essential biological processes such as cell cycle regulation, differentiation, migration, tissue remodeling, neuronal growth, ovulation, and apoptosis.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C8696-25UG | 04061833384374 |
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At what temperature is it shipped? How to reconstitute it? Is it active or to be activated?
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