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C6286

Cathepsin B from bovine spleen

lyophilized powder, ≥10 units/mg protein

Synonym(s):

Cathepsin B1

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10 units
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CLP 162,000
25 units
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CLP 307,000
100 units
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CLP 923,000

About This Item

CAS Number:
9047-22-7
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
3.4.22.1 (BRENDA, IUBMB)
MDL number:
MFCD02662345
Specific activity:
≥10 units/mg protein
Biological source:
bovine spleen

CLP 162,000

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biological source

bovine spleen

Quality Segment

200

form

lyophilized powder

specific activity

≥10 units/mg protein

composition

Protein, ≥30% biuret

UniProt accession no.

P07688

storage temp.

−20°C

Gene Information

cow ... CTSB(281105)

General description

Cathepsin B is a lysosomal enzyme that comprises a light chain (Lys1–Arg49) and a heavy chain (Val50–Thr253).[1] It belongs to cysteine family C1.[2]

Application

Cathepsin B from bovine spleen has been used in cleavage assay before high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis.[3] It has also been used in the enzyme inhibition assays with protease inhibitors RfIP1[4] and ruthenium metalloarenes.[5]

Biochem/physiol Actions

Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis.[4] It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption.[1]
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.
Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

Physical form

Lyophilized powder containing sodium phosphate, sodium chloride and ~6% EDTA as stabilizer.

Other Notes

One unit will hydrolyze 1 μmole of Z-lysine p-nitrophenyl ester per min at pH 5.0 at 25 °C.

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This Item
C0150C8571C3138
Cathepsin B from bovine spleen lyophilized powder, ≥10 units/mg protein

Sigma-Aldrich

C6286

Cathepsin B from bovine spleen

Cathepsin B from human placenta lyophilized powder, ≥5 units/mg protein

Sigma-Aldrich

C0150

Cathepsin B from human placenta

Cathepsin B from human liver buffered aqueous solution, ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)

Sigma-Aldrich

C8571

Cathepsin B from human liver

Cathepsin D from bovine spleen lyophilized powder, ≥2.0 units/mg protein

Sigma-Aldrich

C3138

Cathepsin D from bovine spleen

biological source

bovine spleen

biological source

-

biological source

human liver

biological source

bovine spleen

specific activity

≥10 units/mg protein

specific activity

≥5 units/mg protein

specific activity

≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)

specific activity

≥2.0 units/mg protein

Gene Information

cow ... CTSB(281105)

Gene Information

human ... CTSB(1508)

Gene Information

human ... CTSB(1508)

Gene Information

cow ... CTSD(282883)

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

UniProt accession no.

P07688

UniProt accession no.

P07858

UniProt accession no.

P07858

UniProt accession no.

P80209

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000439495
0000439491
0000439490
0000439490
0000439491

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Global Trade Item Number

SKUGTIN
C6286-10UN04061833511091
C6286-25UN04061833511107
C6286-100UN04061833629178

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