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Merck

B6404

BCIP®/NBT solution, premixed

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About This Item

NACRES:
NA.32
UNSPSC Code:
41105333
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form

liquid

Quality Level

General description

Substrate for visualizing alkaline phosphatase conjugates in Western blotting.

Application

BCIP/NBT solution, premixed has been used:
  • as a substrate to develop dot-blots
  • as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis
  • to detect specific immune complexes in western blot analysis

Other Notes

0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany


Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Articles

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.


Willie Lin et al.
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CD34 is a transmembrane phosphoglycoprotein used to selectively enrich bone marrow in hematopoietic stem cells for transplantation. Treating rats with CD34
Dietary iron concentration may influence aging process by altering oxidative stress in tissues of adult rats
Arruda L F, et al.
PLoS ONE, 8(4), e61058-e61058 (2013)
Neuroprotective effects of recombinant human granulocyte colony-stimulating factor (G-CSF) in neurodegeneration after optic nerve crush in rats
Tsai R K, et al.
Experimental Eye Research, 87(3), 242-250 (2008)



Global Trade Item Number

SKUGTIN
B6404-100ML04061838261533