DNTPM-RO

PCR Nucleotide Mix

solution, sufficient for 500 reactions (11581295001), sufficient for 5,000 reactions (11814362001), sufficient for 2,500 reactions (04638956001)

• NACRES: NA.21
• UNSPSC Code: 41106300
• Assay: 99.8% (sum of 4 peaks, HPLC)
• Form: solution
• Solubility: water: miscible
• Storage temp.: −20°C

Properties

• grade: Molecular Biology
• assay: 99.8% (sum of 4 peaks, HPLC)
• form: solution
• usage: sufficient for 2,500 reactions (04638956001), sufficient for 5,000 reactions (11814362001), sufficient for 500 reactions (11581295001)
• packaging: pkg of 10 × 200 μL (11814362001 [10mM, each]), pkg of 200 μL (11581295001 [10 mM, each]), pkg of 5 × 200 μL (04638956001 [10 mM, each])
• manufacturer/tradename: Roche
• concentration: 10 mM
• impurities: DNase, none detected, RNase, none detected
• color: colorless
• solubility: water: miscible
• storage temp.: −20°C

Description

General description

PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.

Application

• The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions: PCR[1]
• RT-PCR[2]
• cDNA synthesis
• Primer extension

Features and Benefits

The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.

Improve yield and performance

• Choose a convenient ready-to-use mix of all 4 nucleotides.
• Achieve the highest PCR and RT-PCR sensitivity.
• Insist on highest purity (>99%) nucleotides.

Packaging

1 set containing 4 ready-to-use mixes

Preparation Note

Activator: Mg²⁺
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.

Analysis Note

Function Testing in PCR
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• dATP, PCR Grade, sodium salt 10 mM
• dCTP, PCR Grade, sodium salt 10 mM
• dGTP, PCR Grade, sodium salt 10 mM
• dTTP, PCR Grade, sodium salt 10 mM

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: does not flash
• flash_point_c: does not flash