Ultra-High Performance Liquid Chromatography (UHPLC) Method for the Determination of Limonene in Sweet Orange (Citrus sinensis) Oil: Implications for Limonene Stability.

Ultra-High Performance Liquid Chromatography (UHPLC) Method for the Determination of Limonene in Sweet Orange (Citrus sinensis) Oil: Implications for Limonene Stability.

Journal of AOAC International (2015-04-11)

Matthew W Bernart

ABSTRACT

The citrus-derived bioactive monoterpene limonene is an important industrial commodity and fragrance constituent. An RP isocratic elution C18 ultra-HPLC (UHPLC) method using a superficially porous stationary phase and photodiode array (PDA) detector has been developed for determining the limonene content of sweet orange (Citrus sinensis) oil. The method is fast with a cycle time of 1.2 min, linear, precise, accurate, specific, and stability indicating, and it satisfies U.S. Pharmacopeia suitability parameters. The method may be useful in its present form for limonene processing, or modified for research on more polar compounds of the terpenome. A forced-degradation experiment showed that limonene is degraded by heat in hydro-ethanolic solution. PDA detection facilitates classification of minor components of the essential oil, including β-myrcene.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

34851

Sigma-Aldrich

Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%

E7023

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, for molecular biology

459844

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%

34998

Sigma-Aldrich

Acetonitrile, HPLC Plus, ≥99.9%

271004

Sigma-Aldrich

Acetonitrile, anhydrous, 99.8%

493546

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, meets USP testing specifications

360457

Sigma-Aldrich

Acetonitrile, ACS reagent, ≥99.5%

34888

Sigma-Aldrich

Acetonitrile, for HPLC, for UV, ≥99.9% (GC)

439134

Sigma-Aldrich

Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%

51976

Sigma-Aldrich

Etanolo, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)

02870

Sigma-Aldrich

Etanolo, ACS reagent, prima fine spirit, without additive, F15 o¹

183164

Sigma-Aldrich

(R)-(+)-Limonene, 97%

02856

Sigma-Aldrich

Etanolo, purum, absolute ethanol, denaturated with 4.8% isopropanol, A15 IPA¹, ≥99.8% (based on denaturant-free substance)

34881

Sigma-Aldrich

Acetonitrile, suitable for HPLC-GC, ≥99.8% (GC)

493511

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

494445

Sigma-Aldrich

Acetonitrile, biotech. grade, ≥99.93%

W263303

Sigma-Aldrich

(R)-(+)-Limonene, ≥93%

02857

Sigma-Aldrich

Etanolo, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL¹, ~96% (based on denaturant-free substance)

110086

Sigma-Aldrich

Acetonitrile, ReagentPlus^®, 99%

PHR1070

Supelco

Etanolo, Pharmaceutical Secondary Standard; Certified Reference Material

02851

Sigma-Aldrich

Etanolo, for residue analysis

62118

Supelco

(R)-(+)-Limonene, analytical standard

02882

Sigma-Aldrich

Etanolo, purum, absolute ethanol, denaturated with 2% 2-butanone, A15 MEK¹, ≥99.8% (based on denaturant-free substance)

733466

Sigma-Aldrich

Acetonitrile, electronic grade, 99.999% trace metals basis

PHR1551

Supelco

Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material

34442

Sigma-Aldrich

Acetonitrile, suitable for DNA synthesis, ≥99.9% (GC)

1012772

USP

Etanolo, United States Pharmacopeia (USP) Reference Standard

02890

Sigma-Aldrich

Etanolo, purum, fine spirit, denaturated with 2% 2-butanone, F25 MEK¹, ~96% (based on denaturant-free substance)

02858

Sigma-Aldrich

Etanolo, purum, absolute ethanol, denaturated with 1% cyclohexane, A15 CYCLO¹, ≥99.8% (based on denaturant-free substance)

60004

Sigma-Aldrich

Acetonitrile, ≥99.5% (GC)