Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Bio-medical materials and engineering (2014-09-18)

Wenfang Zhao, Kehai Liu, Shunsheng Chen, Man Man Zhu, Hui Lv, Jing Hu, Yuan Mao

ABSTRACT

To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

E7023

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, for molecular biology

34856

Sigma-Aldrich

Diclorometano, suitable for HPLC, ≥99.8%, contains amylene as stabilizer

459844

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%

319902

Sigma-Aldrich

Etilacetato, ACS reagent, ≥99.5%

D65100

Sigma-Aldrich

Diclorometano, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%

34858

Sigma-Aldrich

Etilacetato, suitable for HPLC, ≥99.7%

650463

Sigma-Aldrich

Diclorometano, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 50-150 ppm amylene as stabilizer

650528

Sigma-Aldrich

Etilacetato, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%

493546

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, meets USP testing specifications

270709

Sigma-Aldrich

Benzene, suitable for HPLC, ≥99.9%

319953

Sigma-Aldrich

Benzene, ACS reagent, ≥99.0%

130672

Sigma-Aldrich

N-Hydroxysuccinimide, 98%

32222

Sigma-Aldrich

Diclorometano, puriss. p.a., ACS reagent, reag. ISO, ≥99.9% (GC)

320269

Sigma-Aldrich

Diclorometano, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

33211

Sigma-Aldrich

Etilacetato, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

439169

Sigma-Aldrich

Etilacetato, suitable for HPLC, ≥99.8%

02870

Sigma-Aldrich

Etanolo, ACS reagent, prima fine spirit, without additive, F15 o¹

02856

Sigma-Aldrich

Etanolo, purum, absolute ethanol, denaturated with 4.8% isopropanol, A15 IPA¹, ≥99.8% (based on denaturant-free substance)

90335

Sigma-Aldrich

Trietilammina, BioUltra, ≥99.5% (GC)

330752

Sigma-Aldrich

Triphosgene, reagent grade, 98%

32212

Sigma-Aldrich

Benzene, puriss. p.a., reag. Ph. Eur., ≥99.7%

24233

Sigma-Aldrich

Diclorometano, puriss., meets analytical specification of Ph. Eur., NF, ≥99% (GC)

676853

Sigma-Aldrich

Diclorometano, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

270997

Sigma-Aldrich

Diclorometano, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

493511

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

471283

Sigma-Aldrich

Trietilammina, ≥99.5%

02857

Sigma-Aldrich

Etanolo, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL¹, ~96% (based on denaturant-free substance)

PHR1557

Supelco

Diclorometano, Pharmaceutical Secondary Standard; Certified Reference Material

L092004

Sigma-Aldrich

Etilacetato

W241407

Sigma-Aldrich

Etilacetato, ≥99%, FCC, FG