- Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids.
Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids.
STAR protocols (2020-12-31)
Maria C Heinz, Koen C Oost, Hugo J G Snippert
PMID33377020
ABSTRACT
Patient-derived organoids maintain functional and phenotypic characteristics of the original tissue such as cell-type diversity. Here, we provide protocols on how to label intestinal (cancer) stem cells by integrating the stem cell ASCL2 reporter (STAR) into human and mouse genomes via two different strategies: (1) lentiviral transduction or (2) transposon-based integration. Organoid technology, in combination with the user-friendly nature of STAR, will facilitate basic research in human and mouse adult stem cell biology. For complete details on the use and execution of this protocol, please refer to Oost et al. (2018).
MATERIALI
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Descrizione del prodotto
Sigma-Aldrich
Terreno Eagle modificato di Dulbecco - alto glucosio, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Nicotinamide, BioReagent, suitable for cell culture, suitable for insect cell culture