581301-U
Ascentis® C18 (3 µm) HPLC Columns
L × I.D. 10 cm × 2.1 mm, HPLC Column
Sinonimo/i:
Ascentis RP18 HPLC Column
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About This Item
Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Prodotti consigliati
product name
Colonna per HPLC Ascentis® C18, 3 μm particle size, L × I.D. 10 cm × 2.1 mm
Materiali
stainless steel column
agenzia
suitable for USP L1 (Similar to Phenomenex Luna C18)
Nome Commerciale
Ascentis®
Caratteristiche
endcapped
Produttore/marchio commerciale
Ascentis®
Confezionamento
1 ea of
Grado di funzionalizzazione
25% Carbon loading
Parametri
≤70 °C temp. range
400 bar pressure (5801 psi)
tecniche
HPLC: suitable
LC/MS: suitable
Lungh. × D.I.
10 cm × 2.1 mm
Area superficiale
450 m2/g
Copertura della superficie
3.7 μmol/m2
Impurezze
<5 ppm metals
Matrice
fully porous particle
silica gel high purity, spherical
Gruppo funzionale matrice
C18 (octadecyl) phase
Dimensione particelle
3 μm
Dimensione pori
100 Å
operating pH range
2-8
applicazioni
food and beverages
Tecnica di separazione
reversed phase
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Categorie correlate
Descrizione generale
La famiglia di colonne Ascentis rappresenta la quarta generazione nella tecnologia delle colonne HPLC realizzate dai ricercatori Supelco. Tutte le colonne Ascentis sono caratterizzate da silice da 100 Angstrom a elevata purezza con particelle da 3, 5 e 10 micron. Le colonne sono progettate per applicazioni su piccole molecole e sono adeguabili a varie dimensioni, da quelle delle microcolonne (DI di 1,0 mm) a quelle preparatorie (DI di 50 mm). La famiglia include fase C18, C8, fenil, Si e con gruppo polare integrato, RP-Amide.
Ascentis C18 è una colonna HPLC di prima scelta estremamente stabile e affidabile che fornisce una forma a picchi simmetrici e un′ottima ritenzione anche con composti difficili.
Ascentis C18 è una colonna HPLC di prima scelta estremamente stabile e affidabile che fornisce una forma a picchi simmetrici e un′ottima ritenzione anche con composti difficili.
Applicazioni
- Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: This study employs the Ascentis® C18 HPLC column for the separation of proteotypic tryptic peptides, allowing the identification of human insulin and biosynthetic insulin analogues through multiplexed targeted unlabeled mass spectrometry. The method demonstrated high specificity and sensitivity, enhancing the accuracy of insulin analysis in pharmaceutical research (Qasem et al., 2019).
- Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben, and propylparaben in a topical cream preparation.: Utilizing the Ascentis® C18 HPLC column, this research presents a novel stability-indicating method for the simultaneous assay of multiple compounds in a topical cream. The validated method offers robust stability assessment, critical for ensuring the quality and efficacy of pharmaceutical formulations (Byrne et al., 2014).
- HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: This paper describes the use of the Ascentis® C18 HPLC column in HPLC-DAD and HPLC-ESI-MS/MS methods to profile metabolites in propolis extracts. The column′s performance in separating complex mixtures highlights its utility in natural product research and quality control (Pellati et al., 2011).
- Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: This study compares the performance of the Ascentis® C18 HPLC column with other chromatographic columns for fast separations in pharmaceutical process development. The Ascentis® column provided superior separation efficiency and speed, making it advantageous for high-throughput pharmaceutical analysis (Abrahim et al., 2010).
Caratteristiche e vantaggi
- Ottima ritenzione
- Forma a picchi simmetrici
- Elevata riproducibilità
- Totale compatibilità LC-MS
Note legali
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Applicazioni
N° Catalogo
Descrizione
Determinazione del prezzo
Precolonna
Prodotti correlati
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
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