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Ascentis® Express 90 Å HILIC (5 μm) HPLC Columns

L × I.D. 25 cm × 3 mm, HPLC Column

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

product name

Ascentis® Express HILIC, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm

Materiali

stainless steel column

Livello qualitativo

100

agenzia

suitable for USP L3

Nome Commerciale

Ascentis®

Caratteristiche

endcapped: no

Produttore/marchio commerciale

Ascentis®

Confezionamento

1 ea of

Parametri

60 °C temp. range
600 bar max. pressure (9000 psi)

tecniche

HPLC: suitable
LC/MS: suitable

Lungh. × D.I.

25 cm × 3 mm

Area superficiale

90 m2/g

Impurezze

<5 ppm metals

Matrice

Fused-Core particle platform
superficially porous particle

Gruppo funzionale matrice

silica phase

Dimensione particelle

5 μm

Dimensione pori

90 Å pore size

pH di lavoro

1-8

applicazioni

food and beverages

Tecnica di separazione

hydrophilic interaction (HILIC)
normal phase

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Descrizione generale

Ascentis Express 5 μm HILIC is a high-speed, high-performance liquid chromatography column based on a 5-micron Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shorter diffusion paths in the 0.6-micron thick porous shell around and an overall particle size of ~5-microns. The bare silica stationary phase of Ascentis Express 5 μm HILIC provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.

Column Characteristics
The Fused-Core particle has a surface area of ~ 100 m2/g and an average pore size of 90Å. The Fused-Core particles are 30% to 50% heavier than commercially available totally porous particles due to the density of the solid cores. Therefore, the effective surface area per column is similar to columns packed with totally porous particles having surface areas in the 160-200 m2/g range.

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Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
USRFB001258
USRFB001256
USRFB001254

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Paweł Kubica et al.
Journal of pharmaceutical and biomedical analysis, 127, 184-192 (2016-01-20)
Hydrophilic interaction liquid chromatography (HILIC) coupled with tandem mass spectrometry (MS/MS) was used to separate artificial and natural sweeteners approved for use in European Union (EU). Among three tested HILIC columns (BlueOrchid PAL-HILIC, Ascentis Express Si and Acclaim™ Trinity™ P2)
Tiziana Bertolini et al.
Journal of chromatography. A, 1365, 131-139 (2014-09-23)
A simple, sensitive and fast hydrophilic interaction liquid chromatography (HILIC) method using ultraviolet diode-array detector (UV-DAD)/electrospray ionization tandem mass spectrometry was developed for the automated high performance liquid chromatography (HPLC) determination of sodium risedronate (SR) and its degradation products in
Imran Ali et al.
Biomedical chromatography : BMC, 26(8), 1001-1008 (2012-01-13)
Superficially porous silica particles columns (SPSPCs) are manufactured by different companies. The most common have the brand names Halo, Ascentis Express and Kinetex. These columns provide super fast, sharp peaks and moderate sample loading and back pressure. These are available
Jan Soukup et al.
Journal of chromatography. A, 1374, 102-111 (2014-12-30)
Excess adsorption of water from aqueous acetonitrile mobile phases was investigated on 16 stationary phases using the frontal analysis method and coulometric Karl-Fischer titration. The stationary phases include silica gel and silica-bonded phases with different polarities, octadecyl and cholesterol, phenyl
Alexandre Grand-Guillaume Perrenoud et al.
Journal of chromatography. A, 1360, 275-287 (2014-08-19)
Superficially porous particles (SPP), or core shell particles, which consist of a non-porous silica core surrounded by a thin shell of porous silica, have gained popularity as a solid support for chromatography over the last decade. In the present study

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