There are no available papers that cite using clone VIN-11-5 with Drosophila vinculin. The specific epitope for this antibody has not been mapped. However, there is a paper that maps the epitope binding area of this antibody clone - JBC 266, 8734-8740. 1991, Fusun Kilic, et al. --> "Hence the epitope lies between cysteines 545 and 737." Please see the link below to review this publication:
https://www.sciencedirect.com/science/article/pii/S0021925818315072?via%3Dihub
V4505
Anti-Vinculin Antibody
mouse monoclonal, VIN-11-5
Sinonimo/i:
Vinculin Antibody Sigma, Anti-VCL
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CHF 432.00
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CHF 570.00
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Per il tuo target è disponibile un anticorpo ricombinante privo di conservanti. Prova ZMS1086
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Cambia visualizzazione
100 μL
CHF 432.00
0.2 ML
CHF 570.00
About This Item
CHF 432.00
Check Cart for Availability
Per il tuo target è disponibile un anticorpo ricombinante privo di conservanti. Prova ZMS1086
Richiedi un ordine bulk
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Nome del prodotto
Monoclonal Anti-Vinculin antibody produced in mouse, clone VIN-11-5, ascites fluid
Origine biologica
mouse
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
ascites fluid
Tipo di anticorpo
primary antibodies
Clone
VIN-11-5, monoclonal
PM
antigen 116 kDa
Reattività contro le specie
bovine, human, chicken, mouse
tecniche
indirect immunofluorescence: 1:50 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable
Isotipo
IgG1
N° accesso UniProt
applicazioni
research pathology
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... VCL(7414)
mouse ... Vcl(22330)
Descrizione generale
Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The vinculin (VCL) gene containing 22 exons is mapped to human chromosome 10q22.1-q23. The gene codes for a 117kDa cytoskeletal protein present in adherens type cell junctions. VCL is a ubiquitously expressed cell-adhesion protein. The protein is characterized with a globular head and a tail domain, which are interconnected by a short proline-rich sequence, an intramolecular interaction between the head and tail conceal many ligand-binding sites in the protein.
Specificità
The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.
Immunogeno
smooth muscle vinculin from chicken gizzard.
Applicazioni
Monoclonal Anti-Vinculin antibody produced in mouse has been used in:
- western blotting.
- immunofluorescence.[1]
- immunocytochemistry.
Azioni biochim/fisiol
Vinculin (VCL) is a cytoskeletal protein, which plays a vital role in regulation of cell-to-cell and cell to matrix junctions. VCL facilitates normal embryonic development and cardiac function. Aberration or mutation of the gene leads to cardiomyopathy. VCL also plays a crucial role in cell migration and morphogenesis. VCL might facilitate the binding of integrins and cadherins to the actin cytoskeleton. Vinculin plays an important role in integrin αIIbβ3 signaling and function, which is required for platelet aggregation.
Vinculin is suggested to be a possible link between ends of the bundles of actin filaments and the plasma membrane. It has also been proposed that vinculin may be involved in the transmembrane induction of actin bundle formation.
Altre note
This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200729 Anti-Vinculin antibody, Mouse monoclonal
clone VIN-11-5, purified from hybridoma cell culture
SAB4200729 Anti-Vinculin antibody, Mouse monoclonal
clone VIN-11-5, purified from hybridoma cell culture
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I clienti hanno visto anche
Three-dimensional structure of vinculin bound to actin filaments
Janssen MEW, et al.
Molecular Cell, 21(2), 271-281 (2006)
E P Moiseyeva et al.
The Journal of biological chemistry, 268(6), 4318-4325 (1993-02-25)
The human vinculin gene contains 22 exons ranging in size from 71 base pairs (bp) to 303 bp (average 155 bp) with the exception of exon 22 which contains 144 bp of coding sequence and 1848 bp of 3'-untranslated sequence
Meng Chen et al.
The Journal of biological chemistry, 288(48), 34647-34657 (2013-10-24)
The mylk1 gene encodes a 220-kDa nonmuscle myosin light chain kinase (MLCK), a 130-kDa smooth muscle MLCK (smMLCK), as well as the non-catalytic product telokin. Together, these proteins play critical roles in regulating smooth muscle contractility. Changes in their expression
Liyong Zhang et al.
Nature communications, 5, 3430-3430 (2014-03-13)
The HECT E3 ubiquitin ligase HACE1 is a tumour suppressor known to regulate Rac1 activity under stress conditions. HACE1 is increased in the serum of patients with heart failure. Here we show that HACE1 protects the heart under pressure stress
Qin Yu et al.
Scientific reports, 5, 15245-15245 (2015-10-22)
Hypertension is the most common and lethal risk factor for cardiovascular disease (CVD). Numerous variants have been associated with hypertension, however, most of which failed to get replication due to ethnic differences. In this study, we analyzed associations of 10
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Hello! Has it been published or predicted if this antibody will react with Drosophila vinculin? Or, could you provide where in the human vinculin this antibody interacts?
1 answer-
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