immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
Anti-VP16 is produced in rabbit using a synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue. Whole antiserum is fractionated and further purified by ionexchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Immunogeno
synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue.
Applicazioni
Anti-VP16 recognizes VP16 fusion proteins by immunoblotting and immunoprecipitation. It is used to study the effect of sialic acid on herpes simplex virus type 1 envelope glycoproteins.It is also used to study if self-association of lymphocytic choriomeningitis virus nucleoprotein is mediated by its N-terminal region and is not required for its anti-interferon function.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Health care's future.
R J Nowak
Ohio medicine : journal of the Ohio State Medical Association, 87(5), 229-230 (1991-05-01)
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