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H9658
Anticorpo monoclonale Anti-HA
clone HA-7, ascites fluid
Sinonimo/i:
Anti-HA, Anti-emoagglutinina dell’influenza
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100 μL
CHF 570.00
0.2 ML
CHF 733.00
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Cambia visualizzazione
100 μL
CHF 570.00
0.2 ML
CHF 733.00
About This Item
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
ascites fluid
Tipo di anticorpo
primary antibodies
Clone
HA-7, monoclonal
contiene
15 mM sodium azide
tecniche
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein
Isotipo
IgG1
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Descrizione generale
The gene encoding influenza virus hemagglutinin (HA) precursor is mapped on segment 4. It encodes a 76kDa surface glycoprotein which exists as trimers on the viral envelope.
Specificità
L′anticorpo riconosce le forme native come quelle denaturate-ridotte delle proteine marcate con HA ed è reattivo con le proteine di fusione marcate con HA N- o C-terminale in E. coli o in cellule di mammifero.
Immunogeno
peptide sintetico corrispondente a un frammento dell′emoagglutinina (HA) del virus dell′influenza umana noto come HA-tag, coniugato con KLH
Applicazioni
Monoclonal Anti-HA antibody produced in mouse has been used in 293T cells for:
- immunoprecipitation in human embryonic kidney 293 cells
- immunoblotting in HeLa cells
- immunopurification in human embryonic kidney 293 cells
- immunostaining for electron microscopy in mouse embryonic fibroblasts
- in immunocytochemistry of hippocampal slices
Monoclonal Anti-HA antibody produced in mouse has been used in:
- Immunoblotting, to study the expression of fusion protein
- Coimmunoprecipitation assay
- Immunoblotting of lysate protein
Azioni biochim/fisiol
Hemagglutinin (HA) is the surface glycoprotein of influenza virus. HA is crucial for virus entry and interaction with N-acetylneuraminic acid (Neu5Ac) in host cell surface. HA coordinates with neuraminidase (NA) and facilitates viral entry into host. HA exists as a trimer and each monomer has stalk transmembrane and globular regions. The globular region contains carbohydrate side chains crucial for infecting host.
Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype play a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic.
Stoccaggio e stabilità
For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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What is the Department of Transportation shipping information for this product?
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What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
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Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
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What is the recommended dilution of Product H9658 - HA antibody for immunoprecipitation and for indirect immunostaining?
1 answer-
H9658 - Monoclonal Anti-HA antibody produced in mouse is the ascites fluid. It is not purified. We only test this product in western blotting. This product contains 15-55 mg/ml total protein with 4-7 mg/ml specific antibody. H3663 - Monoclonal Anti-HA antibody produced in mouse as the purified immunoglobulin. We test this product for immunoprecipitation using 0.5-2.0 μg and for immunocytochemistry using 1.0-2.0 μg/mL.These ranges would be a good starting concentration. In order to obtain best results in different techniques and preparations, we recommend determining optimal working dilution by titration test.
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